Stabilization of protein-latex complexes at high ionic strength
نویسنده
چکیده
When studying the colloidal stability of an immunolatex formed by F(ab')2 protein fragments and a polystyrenepolychloromethylstyrene copolymer, striking results were obtained. After the expected particle aggregation induced by an increase in the electrolyte concentration (KBr), subsequent restabilization occurred when this electrolyte concentration was increased even more. This non-DLVO behaviour was studied under different experimental conditions (ionic strength, pH and surface coverage) and with a different solid support (polystyrene) and covering protein (bovine serum albumin) in order to generalize to other systems. An explanation based on ion hydration layer, similar to that proposed for hydrophilic surfaces, is assumed.
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